Saturday, January 18, 2014

Steel Yourself. Silk Worms Are Spinning the Army's Armor


Silk is a highly sought after, gloriously soft fabric. For centuries it has made its way into the hands of artisans, courts of kings, and closets of the rich and famous. But where does this substance come from? For those of you who get squeamish around entomology you probably aren’t going to like this, but you’re technically wearing worm juice. More technically its cocoon, produced in the salivary glands. In other words, spit.
There are a few kinds of silk worms out there but the one that we’re going to be talking about today isn’t one you’ll find in the wild. In fact, most silk worms that are harvested are usually called Bombyx Mori, or domesticated silk worms, so it’s rare you’d find those in the wild either. Although if you find one of these guys pictured above and it has red eyes, you might want to contact University of Wyoming, and quick. You may have a body armor builder on your hands.
So what’s so special about worms and silk? Well I’m glad you asked. Researchers at University of Wyoming are currently marketing a new kind of silk. Well, it’s not a new kind of silk, so much as it’s a new way of spinning it. Using genetics scientists have found a way to splice in the genes that produce Dragline silk in spiders, into the genetic structure of worms. (We’ve talked about genetics a little bit before here at TI&IT. You can read the past article “PhotoshoppingDNA: The Art of Molecular Editing”)
Now this may not sound like such a big deal until you find out that harvesting this silk in mass quantities from a spider isn’t an option. We’ll talk about why they can’t do that today, as well as these neat little pet worms that blossom into beautiful Silk Moths. We’ll also break down the how and why of their silk spinning abilities, a little bit about the history of silk, the reasons they don’t use goats to produce steel thread (Yes…you read that correctly) anymore, and the crazy little proteins that would make Spider-Man drool.

That’s One Shiny Loincloth


Okay, so that’s not a loincloth, but it is really old, and at one time if you had held it up to the light, it would have had a shimmering effect like a diamond. Back then they probably chalked the phenomena up to the Gods…or something else along those lines as the reasons this happens would not have been understood. (And if they were nobody decided to share it.) But however they figured out it occurred it certainly wasn’t divine intervention.
What the effect actually is attributed to is very similar to gem stones referred to as “Cat’s Eye”. It’s called Chatoyancy. While this generally refers to stones cut in a certain way so as to make them more appealing to consumers, it works on silk too. When light hits the surface of the fibers it’s reflected in a triangular-prism format. That’s what gives it the shimmering effect as you move it around in the light.
The oldest silks can be traced back to between 3000 and 4000 B.C.  Do you know where they came from? China of course! For a few thousand years China held dominance on the silk market. That was until the Silk Road opened up and other countries across the world got into the game. Unfortunately though, for the rest of the world silk industry, issues with silkworm disease and production halted the spread of the silk industry across Europe and once again China came out on top.
But how did they figure out that this curious cocoon could be used to clothe the rich and fabulous? According to sources it started with a young Chinese empress named Xi Ling Shi. (Multiple spellings abound all over the Internet) Her palace garden was filled with these trees called Mulberry that silkworms just absolutely adore. Legend says she touched one of the cocoons causing a strand of silk to fall loose. Shortly after the tailors discovered the tensile strength of this textile and decided to put it to use for the royals. Anyone caught in those times smuggling this closely guarded imperial secret was put swiftly to death! (Yikes!)
It wasn’t until the Han Dynasty (206 B.C. – 220 A.D.) that the silk trade really took off. A road was opened up (called the Silk Road by historians surprisingly…) that led them from China to many other nations, starting with Persia and culminating in Europe. They had a hit.

(Land routes are in red, sea trade routes are in blue)

The secret was out. Silk was in. For centuries it dominated the global market’s economy, anybody who was anybody had to get their hands on this strong, soft, pliable material. Its uses ranked from everything to clothing and blankets, drapes and curtains, and many other luxurious items. It took an Empress to figure out that silk was pretty; it took an industry to realize it could make them rich. But how do you get a worm to make something created accidentally as a byproduct of nature by innate self-preservation mechanisms?
                                                                                                    
Make Checks Payable To Wormy, 302 Mulberry Ln.



Left in the wild, silk worms will do their thing. They’ll eat their Mulberry leaves, spin their cocoon, hibernate inside, and through metamorphosis become a flying silk moth. But somewhere along the lines their plans were changed. People began cutting the cocoon’s open, removing the worms, and spinning the delicate fibers into clothing using various machines. Don’t worry about the worm either, they aren’t just thrown away. In fact, most places where silk is harvested, the worms are cooked into various delicacies after de-cocooning them.
If the worms are allowed to enter their moth phase they destroy the silky cocoon on their way out. Proteolytic Enzymes are the reason this happens. It can cause the silk to fall away in strands of random length instead of the cocoon being unraveled as one continuous piece. As you can probably imagine this won’t do for clothing makers. Not only does it shorten the length of the silk strands, it compromises the integrity as well. That’s not good for an industry looking to market silk as some of the most durable stuff around.
Once the cocoon is boiled, de-wormed, and unraveled it’s sent to a machine called a Doubler. This machine does just as the name implies, it doubles the thickness of silk by weaving strands of it together thereby increasing its tensile strength. While silk is strong, it still can break. The Doubler just increases its longevity.
Some of you may be wondering what Tensile strength. Tensile strength just refers to the amount of stress a material can handle before it breaks or snaps. Think in terms of a fishing line, different lines come with different strengths and thicknesses. You wouldn’t go shark fishing with a line rated for Smallmouth Bass. Any kind of material capable of stretching has a tensile strength and, as you can imagine, some are much higher than others.
After the Doubler comes dying the silk. Various Acetic Acid mixtures (found in vinegar) are used to help the dyes bond better to the silk. From there it’s sent to a weaving loom, where it can be spun by a craftsman into a new dress, a soft bed sheet, or even a flowing pair of curtains for your new office room.

(Models wearing dresses spun from fine silks)

But there are other silks out there with higher tensile strengths than that produced by Bombyx mori, (The domesticated silkworm) that are much more highly sought after. Silks produced by the webs of spiders. Yet, it isn’t the clothing industry looking to capitalize on the strong, durable, luxury good. It’s actually the military, and no, not because they want Versace to make uniforms that “pop” and “shimmer”.

Peter Parker’s Haberdashery


Silkworms may have been the first species we commercialized the silk trade through, but Spiders have been doing it better for eons. Spiders produce several kinds of webs. Some are for their internal nests, some are used to catch themselves should they fall, others are used to make the intricate lattices that form their deadly nets, and some are even used to protect their young inside an egg sack. But whatever kind of web the spider spins there is one thing that remains constant. It’s a form of silk.
The strongest silk that a spider is capable of producing is known as Dragline silk. This fiber is so strong that scaled up, it makes Spider-Man seem plausible. (Aside from the radioactive bite to create the acquisition of his powers.) If humans could produce the proteins capable of spinning this material however, and if adjusted for ratios, it would be strong enough to support them. (Think Nylon on steroids)
Dragline silk is used to make the outer connection points for a spider’s web. Because it’s so strong it’s capable of withstanding bombardment from the elements, large prey snags, and constant traversal by our eight-legged arachnids. Some species, such as the orb weaver, have very large abdomens that are relatively weak. They will die if they were to fall from a great height. To avoid this scenario, they use Dragline silk to keep them suspended in the air. Ever seen a spider just dangling there? The fibrous tendril he’s hanging from is what we’re talking about here.
So if it’s so strong, why use silkworms at all? Why not just switch over to using spiders as the main method of harvesting silk? In theory it sounds like a good one but in practice it doesn’t really work so well. See, spiders are extremely territorial, so when one wanders into their neighborhood it becomes a cannibalistic version of the Bloods vs. the Crips. Not a pretty sight. Darn those gangster spiders…
So that rules out spider farms.  
As a result scientists turned to splicing the genes into bacteria. This met with failure. So they tried putting it into Tobacco plants. That didn’t work either. Finally they thought maybe we can put the gene into goats and cows! …I’m sure you can guess that went over like a turd in a punch bowl as well…
Part of the reason it’s so hard to generate spider silk in the lab is that it starts out as a liquid protein that’s produced by a special gland in the spider’s abdomen. Using their spinnerets, spiders apply a physical force to rearrange the protein’s molecular structure and turn it into solid silk. Goats, Tobacco plants, and single-celled organisms can’t do this; they don’t have the biological structure capabilities.

(Spider-Silk “milking” harness)

In 2009, textile expert Simon Peers used 70 people and four years of his life to milk spiders to produce a golden tapestry in Madagascar. While the final result is absolutely gorgeous, (you can read the full article and see what the tapestry looks like here on Wired.) it wasn’t very practical. Nobody wants to wait four years for a rug. It seemed scientists were at an impasse because no other animal had the necessary equipment.
But silkworms do. Silkworms use silk all the time. So if scientists could isolate the right gene for the silk they wanted, maybe they could put it into the body of a worm and it could produce it for them. That was exactly the kind of thinking that led Donald Jarvis, a researcher at University of Wyoming, to this brand new kind of super silk.

Silky Smooth Troops


Because silk is so strong for it’s incredibly miniscule diameters the textile industry wasn’t the only one who wanted to use it. In fact, the military, medical professionals, and architects had their eye on this remarkable material. But before it could be used for these various applications, there first had to be a way to produce it abundantly.
That’s where Jarvis comes in. Using genetics (gotta love genetics) he was able to piggyback the DNA sequence of spiders responsible for creating silk proteins into the makeup of silkworms. Through trial and error they managed to come up with some worms capable of producing various new kinds of silk with even more variable tensile strengths. Just last year this went into production.
But the gene didn’t transfer over to all worms. If there is one thing we know about genes it’s that they are hereditary, which means they are passed on. But not all genes are passed down at once; it seems some of them are selective. (Morgan’s fruit flies anyone?) So how did the scientists determine which worms carried the spider DNA and which ones didn’t?
By using fluorescent dyes they created a mutant worm with glowing red eyes, (that’s a terrifying feature) and used these as an indicator for which ones the gene was present in. After separating the red eyes from the black eyes and breeding them they finally ended up with a stable colony of steel spinning silkworms.
This technology is useful in biodegradable sutures. If you need internal surgery, chances are something inside the body cavity is going to get stitched up. Manufactured sutures, while they can be made biodegradable, still aren’t natural, so harmful chemicals (even though they aren’t deadly ones) get transmuted back into the body. With spider sutures, the proteins will break down naturally and be transformed into other substances the body can either use or safely discard through waste. (Spider poo)
Another particularly interesting use would be for ligament repair. Currently production methods of artificial ligaments are costly, require multiple painful surgeries throughout the patient’s life, and lack the tensile abilities of the real thing. Spider silk on the other hand is extremely pliable, and if woven together into the thickness of a ligament, could require only one surgery to install and last the rest of a patient’s life. Another use they’re looking into is for gauze that can aide in wound healing, (although to be totally honest I’m not sure how that one works.)
While all of these uses will better mankind in the long run, perhaps the shortest-term technological use for this stretchy super string is in body armor.
Currently body armor is bulky, cumbersome, and while it has advanced since the early days of Vietnam, it has a long way to go before it creates perfect protection. Silk is flexible, lightweight, it breathes rather easily and when combined with Dragline Spider-Silk DNA, it’s virtually indestructible. You can see the implications here.
While all these technologies are still in the R&D phase, silk has inadvertently redefined itself and once again is at the top of the pile. Time will tell if we’ll see Spider-men running around the deserts with Orb-Weaver tendons but there is one thing I’m certain of. Science has shown us that silk is much more than just a pretty face.

-       Ryan Sanders



Thanks for reading! If you would like to know more about anything we talked about above in the article feel free to follow any of the links below. Also don’t be afraid to share this around on Facebook and Twitter, and be sure to head over to Facebook and Like To Infinity And…In Theory by clicking here. Thanks again everyone! And happy learning!











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